| Solution Information | help | |
| Enzyme: | Dual specificity mitogen-activated protein kinase kinase 1 [2-31,52-393,S218E,S222D] | |
| inhibitor: | BDBM97653 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | 7.3 | |
| Temp. Prep.: | 25 | |
| Comments: | n/a | |
| pH Buffer 1: | HEPES | |
| Concentration: | 50 nM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 2: | NaCl | |
| Concentration: | 10 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 3: | MgCl2 | |
| Concentration: | 10 mM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 4: | Brij35 | |
| Concentration: | 0.01% | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 5: | MEK1 | |
| Concentration: | 1 nM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 6: | ERK1 | |
| Concentration: | 25 nM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 7: | ATP | |
| Concentration: | 400 uM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 8: | DMSO | |
| Concentration: | 1% | |
| ΔHion: | n/a | |
| Comments: | n/a | |
| pH Buffer 9: | IPTTPITTYFFFK-5FAM-COOH (FI-Erktide) | |
| Concentration: | 500 nM | |
| ΔHion: | n/a | |
| Comments: | n/a | |
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